中国水稻科学 ›› 2017, Vol. 31 ›› Issue (1): 13-22.DOI: 10.16819/j.1001.7216.2017.6101
黄小平1, 张宏玉2, 雷刚1, 王志美1, 章智1, 贺超1, 廖江林1,2,*(), 黄英金1,2,*()
收稿日期:
2016-06-24
修回日期:
2016-09-17
出版日期:
2017-01-20
发布日期:
2017-01-10
通讯作者:
廖江林,黄英金
基金资助:
Xiaoping HUANG1, Hongyu ZHANG2, Gang LEI1, Zhimei WANG1, Zhi ZHANG1, Chao HE1, Jianglin LIAO1,2,*(), Yingjin HUANG1,2,*()
Received:
2016-06-24
Revised:
2016-09-17
Online:
2017-01-20
Published:
2017-01-10
Contact:
Jianglin LIAO, Yingjin HUANG
摘要:
目的 为了阐明水稻灌浆期耐夜间高温的分子机制,提高水稻耐热性,鉴定了水稻近等基因系耐热纯系XN0437T与热敏感纯系XN0437S在灌浆期夜间高温胁迫下的差异表达蛋白质。方法 采用桶栽方法培育水稻,于开花期标记同一天扬花的颖花以保障所取颖花样品的生育进程一致;于水稻灌浆初期(花后第8天)进行夜间高温处理。高温处理结束后剪取带标记的颖花提取水稻籽粒总蛋白质,采用8-plex iTRAQ试剂盒进行蛋白质样品的差异显色标记,标记样品采用高效液相系统进行质谱鉴定及定量分析。结果 鉴定并定量了3130个蛋白质,耐热与热敏感水稻纯系间存在36个差异表达蛋白质。蛋白质功能注释结果显示,鉴定的36个差异表达蛋白质中仅14个蛋白质(占38.9%)注释了功能,12个蛋白质(占33.3%)为推测性功能注释, 10个(占27.8%)为功能未知的蛋白质。已注释功能的14个蛋白质中,5个蛋白质参与能量代谢,3个蛋白质参与物质转运与代谢,2个蛋白质参与光合作用,3个蛋白质为响应逆境的锌指蛋白质,1个蛋白质为响应逆境的热激蛋白质。结论 灌浆期夜间高温影响水稻籽粒细胞内参与能量代谢、物质转运与代谢、光合作用等相关蛋白质的表达模式。水稻籽粒细胞中锌指蛋白质Q67TK9、Q10N88上调表达,锌指蛋白质Q5YLY5下调表达,有利于提高水稻灌浆期对夜间高温的耐热性。
黄小平, 张宏玉, 雷刚, 王志美, 章智, 贺超, 廖江林, 黄英金. 灌浆期夜间高温胁迫下耐热和热敏感水稻籽粒的比较蛋白质组分析[J]. 中国水稻科学, 2017, 31(1): 13-22.
Xiaoping HUANG, Hongyu ZHANG, Gang LEI, Zhimei WANG, Zhi ZHANG, Chao HE, Jianglin LIAO, Yingjin HUANG. Analysis on Comparative Proteomics of Rice Grain Between Heat-tolerant and Heat-sensitive Lines Under High Night Temperature Stress at Filling Stage[J]. Chinese Journal OF Rice Science, 2017, 31(1): 13-22.
蛋白质ID Protein ID | 基因ID Gene ID | HT | HS | HT/HS | 蛋白质的功能描述 Functional illustration of protein |
---|---|---|---|---|---|
能量代谢 Energy metabolism | |||||
Q9FU69 | LOC_Os01g08090 | -1.613 | -2.899 | 1.800 | 糖基转移酶 Glycosyltransferase |
Q7Y179 | LOC_Os03g46660 | 1.794 | 1.000 | 1.793 | 葡聚糖-1,3-β-葡萄糖苷内切酶7 Glucan endo-1,3-beta-glucosidase 7 |
Q0IPF8 | LOC_Os12g10570 | -1.495 | 1.012 | -1.513 | ATP合成酶亚基β ATP synthase subunit beta |
Q2R1N0 | LOC_Os11g38650 | -1.739 | -1.085 | -1.605 | 糖基转移酶 Glycosyltransferase |
Q84T00 | LOC_Os03g58800 | -1.805 | 1.044 | -1.887 | ATP酶家族蛋白质 ATPase, AAA family protein |
物质转运与代谢 Matter translocation and metabolism | |||||
Q8GTK2 | LOC_Os07g46350 | 1.832 | 1.097 | 1.669 | 羧肽酶 Carboxypeptidase |
Q7Y007 | LOC_Os03g47940 | 2.076 | 1.330 | 1.561 | 类似GDSL脂肪酶或酰基水解酶家族蛋白质 GDSL-like Lipase/Acylhydrolase family protein |
Q10BU2 | LOC_Os03g58980 | -1.672 | 1.267 | -2.119 | 类似(种子)萌发蛋白质3-7 Germin-like protein 3-7 |
光合作用 Photosynthesis | |||||
Q2R237 | LOC_Os11g37130 | 3.115 | 1.199 | 2.598 | 叶绿体独立转位酶蛋白质 TATB Sec-independent protein translocase protein TATB, chloroplastic |
Q658I1 | LOC_Os06g03790 | -1.667 | 1.674 | -2.793 | 核糖体蛋白质L29 Ribosomal protein L29 |
逆境应答 Stress response | |||||
Q67TK9 | B1012G11.2 | 2.852 | -1.041 | 2.967 | 锌指蛋白质 Zinc knuckle containing protein-like |
Q10N88 | LOC_Os03g17020 | 2.341 | 1.326 | 1.765 | ZIGA2锌指蛋白质 ARFGAP-like zinc finger-containing protein ZIGA2 |
Q84Q72 | LOC_Os03g16030 | 1.906 | 2.975 | -1.560 | 18.1 kD热激蛋白质类型Ⅰ 18.1 kD class Ⅰ heat shock protein |
Q5YLY5 | LOC_Os06g50840 | -4.167 | -1.560 | -2.667 | 锌指蛋白质 Zinc finger protein-like |
推测性功能注释蛋白质 Preliminary annotation of unknown-function protein | |||||
Q6ZJ47 | LOC_Os08g02550 | 3.150 | -1.522 | 4.796 | 可能的COP9复合亚基3 Putative COP9 complex subunit 3, FUS11 |
Q6ZCC9 | LOC_Os08g02030 | 1.842 | -1.764 | 3.251 | 可能的AER蛋白质 Putative AER |
Q6ZCP8 | LOC_Os08g07060 | 2.046 | -1.477 | 3.020 | 可能的泛素氧化还原酶亚基1 Putative ubiquinone oxidoreductase subunit 1 |
Q6I587 | LOC_Os05g5081 | 2.045 | 1.057 | 1.934 | 可能的钙依赖蛋白质激酶 Putative calcium-dependent protein kinase |
Q53M16 | LOC_Os11g13690 | 1.109 | -1.560 | 1.730 | 假定蛋白质 Hypothetical protein |
Q9LWS6 | LOC_Os06g02470 | 1.699 | -1.003 | 1.704 | 臭氧胁迫响应相关蛋白质 Ozone-responsive stress-related protein-like |
Q7XI37 | LOC_Os07g42890 | 1.918 | 1.150 | 1.668 | 可能的FH互作蛋白质FIP1 Putative FH protein interacting protein FIP1 |
Q69MT6 | LOC_Os09g34140 | 1.908 | 1.158 | 1.649 | 假定蛋白质 Hypothetical protein OSJNBb0034B12.21 |
Q338P8 | LOC_Os10g25010 | 1.570 | 1.013 | 1.550 | 可能的钙离子结合蛋白质 Probable calcium-binding protein CML8 |
Q8H8U1 | LOC_Os03g17510 | 1.038 | 1.574 | -1.517 | 可能的PPR重复蛋白质 Putative PPR repeat containing protein |
Q7XI22 | LOC_Os07g08880 | 1.215 | 1.877 | -1.546 | 可能的ES43蛋白质 Putative ES43 protein |
Q94GQ6 | LOC_Os03g53620 | 1.129 | 1.959 | -1.736 | 可能的脱落酸诱导蛋白质 Putative abscisic acid-inducible protein |
功能未知蛋白质 Unknown-function protein | |||||
C7JA46 | LOC_Os12g44380 | 1.792 | -1.144 | 2.051 | |
Q7XQ85 | LOC_Os04g48850 | -1.015 | -1.894 | 1.864 | |
Q0E0B4 | LOC_Os02g35630 | 1.075 | -1.548 | 1.664 | |
Q7XVN6 | LOC_Os04g32070 | 1.511 | -1.055 | 1.594 | |
Q0E3F2 | LOC_Os02g08130 | 2.037 | 1.282 | 1.589 | |
Q6YUH8 | LOC_Os02g46956 | 1.174 | 1.848 | -1.572 | |
Q0DTK3 | LOC_Os03g13850 | -1.575 | 1.043 | -1.642 | |
Q6ES31 | LOC_Os09g24710 | 1.018 | 1.757 | -1.727 | |
Q5N9C8 | LOC_Os01g51220 | -1.116 | 1.771 | -1.976 | |
Q0E225 | LOC_Os02g17760 | -2.793 | -1.285 | -2.179 |
表1 耐热纯系与热敏感纯系的差异表达蛋白质的表达模式及其功能注释
Table 1 Expression model and functional illustration of the differentially expressed proteins between the heat-tolerant and the heat-sensitive lines.
蛋白质ID Protein ID | 基因ID Gene ID | HT | HS | HT/HS | 蛋白质的功能描述 Functional illustration of protein |
---|---|---|---|---|---|
能量代谢 Energy metabolism | |||||
Q9FU69 | LOC_Os01g08090 | -1.613 | -2.899 | 1.800 | 糖基转移酶 Glycosyltransferase |
Q7Y179 | LOC_Os03g46660 | 1.794 | 1.000 | 1.793 | 葡聚糖-1,3-β-葡萄糖苷内切酶7 Glucan endo-1,3-beta-glucosidase 7 |
Q0IPF8 | LOC_Os12g10570 | -1.495 | 1.012 | -1.513 | ATP合成酶亚基β ATP synthase subunit beta |
Q2R1N0 | LOC_Os11g38650 | -1.739 | -1.085 | -1.605 | 糖基转移酶 Glycosyltransferase |
Q84T00 | LOC_Os03g58800 | -1.805 | 1.044 | -1.887 | ATP酶家族蛋白质 ATPase, AAA family protein |
物质转运与代谢 Matter translocation and metabolism | |||||
Q8GTK2 | LOC_Os07g46350 | 1.832 | 1.097 | 1.669 | 羧肽酶 Carboxypeptidase |
Q7Y007 | LOC_Os03g47940 | 2.076 | 1.330 | 1.561 | 类似GDSL脂肪酶或酰基水解酶家族蛋白质 GDSL-like Lipase/Acylhydrolase family protein |
Q10BU2 | LOC_Os03g58980 | -1.672 | 1.267 | -2.119 | 类似(种子)萌发蛋白质3-7 Germin-like protein 3-7 |
光合作用 Photosynthesis | |||||
Q2R237 | LOC_Os11g37130 | 3.115 | 1.199 | 2.598 | 叶绿体独立转位酶蛋白质 TATB Sec-independent protein translocase protein TATB, chloroplastic |
Q658I1 | LOC_Os06g03790 | -1.667 | 1.674 | -2.793 | 核糖体蛋白质L29 Ribosomal protein L29 |
逆境应答 Stress response | |||||
Q67TK9 | B1012G11.2 | 2.852 | -1.041 | 2.967 | 锌指蛋白质 Zinc knuckle containing protein-like |
Q10N88 | LOC_Os03g17020 | 2.341 | 1.326 | 1.765 | ZIGA2锌指蛋白质 ARFGAP-like zinc finger-containing protein ZIGA2 |
Q84Q72 | LOC_Os03g16030 | 1.906 | 2.975 | -1.560 | 18.1 kD热激蛋白质类型Ⅰ 18.1 kD class Ⅰ heat shock protein |
Q5YLY5 | LOC_Os06g50840 | -4.167 | -1.560 | -2.667 | 锌指蛋白质 Zinc finger protein-like |
推测性功能注释蛋白质 Preliminary annotation of unknown-function protein | |||||
Q6ZJ47 | LOC_Os08g02550 | 3.150 | -1.522 | 4.796 | 可能的COP9复合亚基3 Putative COP9 complex subunit 3, FUS11 |
Q6ZCC9 | LOC_Os08g02030 | 1.842 | -1.764 | 3.251 | 可能的AER蛋白质 Putative AER |
Q6ZCP8 | LOC_Os08g07060 | 2.046 | -1.477 | 3.020 | 可能的泛素氧化还原酶亚基1 Putative ubiquinone oxidoreductase subunit 1 |
Q6I587 | LOC_Os05g5081 | 2.045 | 1.057 | 1.934 | 可能的钙依赖蛋白质激酶 Putative calcium-dependent protein kinase |
Q53M16 | LOC_Os11g13690 | 1.109 | -1.560 | 1.730 | 假定蛋白质 Hypothetical protein |
Q9LWS6 | LOC_Os06g02470 | 1.699 | -1.003 | 1.704 | 臭氧胁迫响应相关蛋白质 Ozone-responsive stress-related protein-like |
Q7XI37 | LOC_Os07g42890 | 1.918 | 1.150 | 1.668 | 可能的FH互作蛋白质FIP1 Putative FH protein interacting protein FIP1 |
Q69MT6 | LOC_Os09g34140 | 1.908 | 1.158 | 1.649 | 假定蛋白质 Hypothetical protein OSJNBb0034B12.21 |
Q338P8 | LOC_Os10g25010 | 1.570 | 1.013 | 1.550 | 可能的钙离子结合蛋白质 Probable calcium-binding protein CML8 |
Q8H8U1 | LOC_Os03g17510 | 1.038 | 1.574 | -1.517 | 可能的PPR重复蛋白质 Putative PPR repeat containing protein |
Q7XI22 | LOC_Os07g08880 | 1.215 | 1.877 | -1.546 | 可能的ES43蛋白质 Putative ES43 protein |
Q94GQ6 | LOC_Os03g53620 | 1.129 | 1.959 | -1.736 | 可能的脱落酸诱导蛋白质 Putative abscisic acid-inducible protein |
功能未知蛋白质 Unknown-function protein | |||||
C7JA46 | LOC_Os12g44380 | 1.792 | -1.144 | 2.051 | |
Q7XQ85 | LOC_Os04g48850 | -1.015 | -1.894 | 1.864 | |
Q0E0B4 | LOC_Os02g35630 | 1.075 | -1.548 | 1.664 | |
Q7XVN6 | LOC_Os04g32070 | 1.511 | -1.055 | 1.594 | |
Q0E3F2 | LOC_Os02g08130 | 2.037 | 1.282 | 1.589 | |
Q6YUH8 | LOC_Os02g46956 | 1.174 | 1.848 | -1.572 | |
Q0DTK3 | LOC_Os03g13850 | -1.575 | 1.043 | -1.642 | |
Q6ES31 | LOC_Os09g24710 | 1.018 | 1.757 | -1.727 | |
Q5N9C8 | LOC_Os01g51220 | -1.116 | 1.771 | -1.976 | |
Q0E225 | LOC_Os02g17760 | -2.793 | -1.285 | -2.179 |
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